Effect of divalent metal ions on nucleotide inhibition of pig heart citrate synthase.
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چکیده
منابع مشابه
Effects of salts and organic solvents on the activity of citrate synthase.
The enzymatic activity of citrate synthase from pig heart can be inhibited by various ions. The order of inhibitory effect of monovalent cations is Li+ > K+ > Na+ > Cs+ and that of anions is SCN> Cl> tartrate. Divalent cations such as Ca2+ and Mg2+ are much more effective than monovalent cations, but thiocyanate is as effective as divalent cations. ATP is also a potent inhibitor, but dATP has n...
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Bacillus subtilis 168 was assayed for its growth on tricarboxylic acid (TCA) cycle intermediates and related compounds as the sole carbon sources. Growth of the organism was supported by citrate, D-isocitrate, succinate, fumarate and L-malate, whereas no growth was observed in the presence of cis-aconitate,2-oxoglutarate, D-malate, oxaloacetate and tricarballylate. Growth of the organism on the...
متن کاملGrowth of Bacillus subtilis on citrate and isocitrate is supported by the Mg-citrate transporter CitM
Bacillus subtilis 168 was assayed for growth on TCA cycle intermediates and related compounds as sole carbon source. Growth was supported by citrate, D-isocitrate, succinate, fumarate and L-malate, whereas no growth was observed in the presence of cis-aconitate, αketoglutarate, D-malate, oxaloacetate and tricarballylate. Growth on the tricarboxylates citrate and D-isocitrate required the presen...
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We have demonstrated that citrate synthase may be assayed by a simple, discontinuous, spectrophotometric procedure based on the measurement of oxaloacetate utilization with 2,4-dinitrophenylhydrazine. The assay is applicable both to the purified enzyme and to cell extracts, and has the advantage that it can be used in the presence of high concentrations of thiols and thioesters. We have used th...
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ورودعنوان ژورنال:
- The Journal of biological chemistry
دوره 241 15 شماره
صفحات -
تاریخ انتشار 1966